解剖学和形态学
麻醉学
听力与言语-语言病理学
行为科学
心脏和心血管系统
细胞和组织工程学
临床神经病学
危重症监护医学
牙科,口腔外科和医学
皮肤病学
急诊医学
内分泌学和新陈代谢
肠胃学和肝脏学
老人病学和老年医学
卫生保健科学和服务
血液学
免疫学
传染病
综合和补充性医学
医学伦理学
医学信息学
医学实验室技术
医学,全科和内科
医学,法律
医学,研究和试验
神经系统科学
护理
营养学和饮食学
产科医学和妇科医学
肿瘤学
眼科学
整形外科学
耳鼻喉科学
病理学
儿科学
周围血管疾病
药理学和药剂学
生理学
基本医疗保健
精神病学
公共、环境和职业卫生
放射学,核医学和医学成像
康复学
生殖生物学
呼吸系统
风湿病学
运动科学
外科学
毒理学
热带医学
泌尿学和肾脏学
病毒学
老年医学
健康政策和服务
心理学,临床
abstract::Apoptosis is initiated when Bcl-2 and its prosurvival relatives are engaged by proapoptotic BH3-only proteins via interaction of its BH3 domain with a groove on the Bcl-2-like proteins. These interactions have been considered promiscuous, but our analysis of the affinity of eight BH3 peptides for five Bcl-2-like prote...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.12.030
更新日期:2005-02-04 00:00:00
abstract::The RhoA GTPase controls many cellular functions, including gene transcription and actin polymerization. Several lines of evidence suggest that Rho GTPases are required for B cell receptor (BCR) signaling, but whether RhoA is necessary has not been investigated. Here, we show that RhoA is activated, downstream of PI3K...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.12.012
更新日期:2005-01-21 00:00:00
abstract::Interference with nucleocytoplasmic transport is a strategy employed by certain viruses to compromise host cellular function. While it has been shown that the matrix (M) protein of the vesicular stomatitis virus (VSV) inhibits nuclear export of host cell mRNAs, the underlying mechanism has not been fully established. ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.11.023
更新日期:2005-01-07 00:00:00
abstract::MicroRNAs have recently emerged as key posttranscriptional regulators of eukaryotic gene expression, yet our understanding of how microRNA expression is itself controlled has remained rudimentary. This review describes recent insights into the mechanisms governing microRNA transcription and processing in vertebrates a...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2004.12.002
更新日期:2004-12-22 00:00:00
abstract::Yeast histone H2A is phosphorylated on Ser129 upon DNA damage, an event required for efficient repair. We show that phosphorylation occurs rapidly over a large region around DNA double-strand breaks (DSBs). Histone H4 acetylation is also important for DSB repair, and we found that the NuA4 HAT complex associates speci...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.12.003
更新日期:2004-12-22 00:00:00
abstract::Nuclear export of mRNA in eukaryotic cells is mediated by soluble transport factors and components of the nuclear pore complex (NPC). The cytoplasmically oriented nuclear pore protein Nup159 plays a critical role in mRNA export through its conserved N-terminal domain (NTD). Here, we report the crystal structure of the...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.10.032
更新日期:2004-12-03 00:00:00
abstract::A remarkable paper from the de Lange lab (Wang et al., 2004) in a recent issue of Cell reveals that homologous recombination can result in the abrupt shortening of telomeres in a process that appears to involve reciprocal crossing over within the t-loop structure that protects chromosome ends. ...
journal_title:Molecular cell
pub_type: 评论,杂志文章
doi:10.1016/j.molcel.2004.11.006
更新日期:2004-11-19 00:00:00
abstract::The DNA polymerase from phage phi29 is a B family polymerase that initiates replication using a protein as a primer, attaching the first nucleotide of the phage genome to the hydroxyl of a specific serine of the priming protein. The crystal structure of phi29 DNA polymerase determined at 2.2 A resolution provides expl...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.10.019
更新日期:2004-11-19 00:00:00
abstract::Serine-arginine (SR) proteins are general splicing factors and can function through binding to exonic splicing enhancers (ESEs). SR proteins and several other mammalian splicing factors contain an arginine-serine-rich (RS) domain required to promote splicing. We have recently found that the ESE bound RS domain functio...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.10.021
更新日期:2004-11-05 00:00:00
abstract::Study of mutant phenotypes is a fundamental method for understanding gene function. The construction of a near-complete collection of yeast knockouts (YKO) and the unique molecular barcodes (or TAGs) that identify each strain has enabled quantitative functional profiling of Saccharomyces cerevisiae. By using these TAG...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.09.035
更新日期:2004-11-05 00:00:00
abstract::Cotranscriptional loading of proteins onto nascent transcripts contributes to the formation of messenger ribonucleoprotein particles (mRNPs) competent for nuclear export. The transcription machinery is believed to play a pivotal role in mRNP assembly, which is at least partially linked to the function of the THO/TREX ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.09.019
更新日期:2004-10-22 00:00:00
abstract::The oncoprotein BCL-3 is a nuclear transcription factor that activates NF-kappaB target genes through formation of heterocomplexes with p50 or p52. BCL-3 is phosphorylated in vivo, but specific BCL-3 kinases have not been identified so far. In this report, we show that BCL-3 is a substrate for the protein kinase GSK3 ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.09.004
更新日期:2004-10-08 00:00:00
abstract::To map the genomic interaction sites of chromatin proteins, two related methods were developed and experimentally explored in Saccharomyces cerevisiae. The ChIC method (chromatin immunocleavage) consists of tethering a fusion protein (pA-MN) consisting of micrococcal nuclease (MN) and staphylococcal protein A to speci...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.09.007
更新日期:2004-10-08 00:00:00
abstract::Death-fold domains constitute an evolutionarily conserved superfamily that mediates apoptotic signaling. These motifs, including CARD (caspase recruitment domain), DD (death domain), and DED (death effector domain), are believed to exert their effects solely through homotypic interactions. Herein we demonstrate that t...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.08.020
更新日期:2004-09-24 00:00:00
abstract::A variety of debilitating diseases including diabetes, Alzheimer's, Huntington's, Parkinson's, and prion-based diseases are linked to stress within the endoplasmic reticulum (ER). Using S. cerevisiae, we sought to determine the relationship between protein misfolding, ER stress, and cell death. In the absence of ERV29...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.08.025
更新日期:2004-09-10 00:00:00
abstract::The activation of NF-kappaB and IKK requires an upstream kinase complex consisting of TAK1 and adaptor proteins such as TAB1, TAB2, or TAB3. TAK1 is in turn activated by TRAF6, a RING domain ubiquitin ligase that facilitates the synthesis of lysine 63-linked polyubiquitin chains. Here we present evidence that TAB2 and...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.08.008
更新日期:2004-08-27 00:00:00
abstract::Recycling of RNA polymerase II (Pol II) after transcription requires dephosphorylation of the polymerase C-terminal domain (CTD) by the phosphatase Fcp1. We report the X-ray structure of the small CTD phosphatase Scp1, which is homologous to the Fcp1 catalytic domain. The structure shows a core fold and an active cent...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.06.035
更新日期:2004-08-13 00:00:00
abstract::Argonaute proteins associate with small RNAs that guide mRNA degradation, translational repression, or a combination of both. The human Argonaute family has eight members, four of which (Ago1 through Ago4) are closely related and coexpressed in many cell types. To understand the biological function of the different Ag...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.07.007
更新日期:2004-07-23 00:00:00
abstract::Analyses of isolated pre-ribosomes yielded biochemical "snapshots" of the dynamic, nascent 60S and 40S subunits during their path from the nucleolus to the cytoplasm. Here, we present the structure of a pre-60S ribosomal intermediate located in the nucleoplasm. A huge dynein-related AAA-type ATPase (Rea1) and the Rix1...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.06.033
更新日期:2004-07-23 00:00:00
abstract::Related coactivators p300 and CBP affect the transcriptional activities of many transcription factors (TF), producing multiple downstream effects. Here we show that immediate early response TF, Egr1, acts upstream of p300/CBP to induce or to repress transcription, depending on the stimulus. Cells induced with serum to...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.06.030
更新日期:2004-07-02 00:00:00
abstract::The abundant chromatin-associated human factor HCF-1 is a heterodimeric complex of HCF-1N and HCF-1C subunits that are essential for two stages of the cell cycle. The HCF-1N subunit promotes G1 phase progression, whereas the HCF-1C subunit ensures proper cytokinesis at completion of M phase. How the HCF-1C subunit fun...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.06.008
更新日期:2004-06-18 00:00:00
abstract::Neurons undertake an amazing journey from the center of the developing mammalian brain to the outer layers of the cerebral cortex. Doublecortin, a component of the microtubule cytoskeleton, is essential in postmitotic neurons and was identified because its mutation disrupts human brain development. Doublecortin stabil...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.06.009
更新日期:2004-06-18 00:00:00
abstract::Prions constitute a rare class of protein, which can switch to a robust amyloid form and then propagate that form in the absence of a nucleic acid determinant, thereby creating a unique, protein-only infectious agent. Details of the mechanism that drives conversion to the prion form and then subsequent propagation of ...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/j.molcel.2004.05.012
更新日期:2004-06-04 00:00:00
abstract::Nucleosome loss from a promoter region has recently been described as a potential mechanism for transcriptional regulation. We investigated whether H3/H4 histone chaperones mediate the loss of nucleosomes from the promoter of the yeast PHO5 gene during transcriptional activation. We found that antisilencing function 1...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/j.molcel.2004.05.016
更新日期:2004-06-04 00:00:00
abstract::Yeast Sir2 protein regulates chromatin structure and suppresses recombination at the multiple tandem rDNA array. In the current issue of Cell, Kobayashi and colleagues reinvestigate rDNA dynamics in sir2 strains and find that Sir2 is necessary to recruit cohesins to the array, which influences the nature of rDNA recom...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/s1097-2765(04)00266-7
更新日期:2004-05-21 00:00:00
abstract::The phosphoinositide (PI)-3-kinase-related kinase (PIKK) family proteins Tel1p and Mec1p have been implicated in the telomere integrity of Saccharomyces cerevisiae. However, the mechanism of PIKK-mediated telomere length control remains unclear. Here, we show that Tel1p and Mec1p are recruited to the telomeres at spec...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00262-x
更新日期:2004-05-21 00:00:00
abstract::All nuclear transcription is interrupted during mitosis. We examined the role of human TTF2, an RNA polymerase (Pol) I and II termination factor, in mitotic repression of transcription elongation. We find that TTF2 levels rise in the cytoplasm in S and G2 and at the onset of mitosis TTF2 translocates into the nucleus....
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00234-5
更新日期:2004-05-07 00:00:00
abstract::The subclass of cysteine proteases termed lysosomal cathepsins has long been thought to be primarily involved in end-stage protein breakdown within lysosomal compartments. Furthermore, few specific protein substrates for these proteases have been identified. We show here that cathepsin L functions in the regulation of...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00209-6
更新日期:2004-04-23 00:00:00
abstract::The C-terminal fragment, Bb, of factor B combines with C3b to form the pivotal C3-convertase, C3bBb, of alternative complement pathway. Bb consists of a von Willebrand factor type A (vWFA) domain that is structurally similar to the I domains of integrins and a serine protease (SP) domain that is in inactive conformati...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00160-1
更新日期:2004-04-09 00:00:00
abstract::Spongiform encephalopathies are believed to be transmitted by a unique mechanism involving self-propagating conformational conversion of prion protein into a misfolded form. Here we demonstrate that fundamental aspects of mammalian prion propagation, including the species barrier and strain diversity, can be reproduce...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00155-8
更新日期:2004-04-09 00:00:00
abstract::The 3D structures of all 12 mispairs formed in the active site of a DNA polymerase help explain their differential effects on polymerase stalling and on translocation of the primer terminus to the enzyme's proofreading site. ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00149-2
更新日期:2004-03-26 00:00:00
abstract::Our previous study showed that ubiquitination of p53 is reversible and that the ubiquitin hydrolase HAUSP can stabilize p53 by deubiquitination. Here, we found that partial reduction of endogenous HAUSP levels by RNAi indeed destabilizes endogenous p53; surprisingly, however, nearly complete ablation of HAUSP stabiliz...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00157-1
更新日期:2004-03-26 00:00:00
abstract::The rtpA gene of Bacillus subtilis encodes the Anti-TRAP protein, AT. AT can bind and inhibit the TRAP regulatory protein, preventing TRAP from promoting transcription termination in the trpEDCFBA operon leader region. AT synthesis is upregulated transcriptionally and translationally in response to the accumulation of...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00085-1
更新日期:2004-03-12 00:00:00
abstract::Salmonella force their way into nonphagocytic host intestinal cells to initiate infection. Uptake is triggered by delivery into the target cell of bacterial effector proteins that stimulate cytoskeletal rearrangements and membrane ruffling. The Salmonella invasion protein A (SipA) effector is an actin binding protein ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00053-x
更新日期:2004-02-27 00:00:00
abstract::Allosteric communication between interacting molecules is fundamental to signal transduction and many other cellular processes. To better understand the relationship between nuclear receptor (NR) ligand positioning and the formation of the coactivator binding pocket, we investigated the determinants of ligand selectiv...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00054-1
更新日期:2004-02-13 00:00:00
abstract::In Saccharomyces cerevisiae, methylation of histone H3 at active genes is an epigenetic mark that distinguishes active from silent chromatin and functions as a short-term "memory" of recent transcription. Methylation of H3 at lysine residues K4 and K79 depends on ubiquitylation of histone H2B, but the mechanisms linki...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(04)00026-7
更新日期:2004-02-13 00:00:00
abstract::Lysine acetylation of the tumor suppressor protein p53 in response to a wide variety of cellular stress signals is required for its activation as a transcription factor that regulates cell cycle arrest, senescence, or apoptosis. Here, we report that the conserved bromo-domain of the transcriptional coactivator CBP (CR...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00528-8
更新日期:2004-01-30 00:00:00
abstract::The protective "cap" that assembles at chromosome ends recruits and controls an intricate network of biochemical activities, each one critical for telomere structure and the maintenance of genomic stability. Recent studies have uncovered the components of telomere caps and have started to define the pathways that lead...
journal_title:Molecular cell
pub_type: 杂志文章,评审
doi:10.1016/s1097-2765(03)00531-8
更新日期:2004-01-16 00:00:00
abstract::Calreticulin and calnexin are homologous lectins that serve as molecular chaperones for glycoproteins in the endoplasmic reticulum of eukaryotic cells. Here we show that calreticulin depletion specifically accelerates the maturation of cellular and viral glycoproteins with a modest decrease in folding efficiency. Caln...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00494-5
更新日期:2004-01-16 00:00:00
abstract::Molecular motors are the long-haul carriers of eukaryotic cells, moving cargos bidirectionally along microtubule tracks. In the December 12th issue of Cell, report that HDAC6, a tubulin deacetylase, functions as an adaptor that links cargos of aggregated protein to the minus end-directed motor, cytoplasmic dynein. ...
journal_title:Molecular cell
pub_type: 杂志文章
doi:10.1016/s1097-2765(03)00498-2
更新日期:2003-12-01 00:00:00